Anticorps Polyclonal de lapin anti-VEGFR2/KDR

• Phare
• Validé par KD/KO

VEGFR2/KDR Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(136)

Avis (3)

N° de cat : 26415-1-AP

Synonymes

KDR, VEGFR2, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor flk-1, FLK-1

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Galerie de données de validation

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  WB analysis using 26415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 26415-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26415-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26415-1-AP (VEGF Receptor 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 26415-1-AP

  IP result of anti-VEGFR2/KDR (IP:26415-1-AP, 4ug; Detection:26415-1-AP 1:3000) with human placenta tissue lysate 600 ug.

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  IP experiment of rat skeletal muscle using 26415-1-AP

  IP result of anti-VEGFR2/KDR (IP:26415-1-AP, 4ug; Detection:26415-1-AP 1:1000) with rat skeletal muscle tissue lysate 760 ug.

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  IHC staining of human placenta using 26415-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26415-1-AP (VEGFR2/KDR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26415-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26415-1-AP (VEGFR2/KDR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu cardiaque de souris, tissu placentaire de souris, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu placentaire humain, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 100 publications below
• IHC: See 19 publications below
• IF: See 37 publications below
• IP: See 2 publications below
• FC: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

26415-1-AP cible VEGFR2/KDR dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEGFR2/KDR Protéine recombinante Ag24589
• Nom complet: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Masse moléculaire calculée: 1356 aa, 152 kDa
• Poids moléculaire observé: 150 kDa, 230 kDa
• Numéro d’acquisition GenBank: BC131822
• Symbole du gène: KDR
• Identification du gène (NCBI): 3791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Publications

Species	Application	Title
human	WB	Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
human	IF	Sci Bull (Beijing); Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes; Authors - Jiangbing Xiang
mouse	WB	J Exp Med; Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy.; Authors - Michelle E LeBlanc
human	WB,IHC	Aging Dis; MSC-Derived Exosomes can Enhance the Angiogenesis of Human Brain MECs and Show Therapeutic Potential in a Mouse Model of Parkinson's Disease.; Authors - Chunling Xue
	WB,IF	J Neuroinflammation; Succinate-induced macrophage polarization and RBP4 secretion promote vascular sprouting in ocular neovascularization; Authors - Tianyi Shen
human	WB	Bioact Mater; Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.; Authors - Wenjing Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Poulomi (Verified Customer) (07-02-2024)

The blot was good. It detected VEGFR2 in brain endothelial cells and Hek cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Endothelial Cells

FH

Sammy (Verified Customer) (01-20-2024)

This works well to detect VEGFR2 in HUVEC lysate. Ab diluted in 3% BSA PBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on angiogenesis study and use this for WB on HUVECs. It works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs

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