Anticorps Monoclonal anti-VEGFR2

VEGFR2 Monoclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1G2A8

N° de cat : 67407-1-Ig

Synonymes

CD309, Fetal liver kinase 1, FLK 1, FLK1, KDR, Kinase insert domain receptor, VEGFR, VEGFR 2, VEGFR2

---

Galerie de données de validation

• 

  WB analysis of human placenta using 67407-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67407-1-Ig (VEGFR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HUVEC using 67407-1-Ig

  1X10^6 HUVEC cells were intracellularly stained with 0.20 ug/test Anti-Human VEGFR2 (67407-1-Ig, Clone:1G2A8) (red) or 0.20 ug Mouse IgG1 Isotype Control(66360-1-Ig, CloneT1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

---

Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en cytométrie: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67407-1-Ig cible VEGFR2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VEGFR2 Protéine recombinante Ag24589
• Nom complet: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Masse moléculaire calculée: 1356 aa, 152 kDa
• Poids moléculaire observé: 230 kDa
• Numéro d’acquisition GenBank: BC131822
• Symbole du gène: KDR
• Identification du gène (NCBI): 3791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Publications

Species	Application	Title
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin
human	WB	Front Genet; ANLN and KDR Are Jointly Prognostic of Breast Cancer Survival and Can Be Modulated for Triple Negative Breast Cancer Control.; Authors - Xiaofeng Dai
human	IHC,IF	Cell Tissue Res; A histological study of vascular wall resident stem cells in venous malformations; Authors - Hou-Fu Xia
human	WB	Phytother Res; Sclareol attenuates liver fibrosis through SENP1-mediated VEGFR2 SUMOylation and inhibition of downstream STAT3 signaling; Authors - Mao-Xu Ge
human	WB	Cell Signal; Exosomal B7-H3 facilitates colorectal cancer angiogenesis and metastasis through AKT1/mTOR/VEGFA pathway; Authors - Rendi Wu
human	WB	Cells; PLAC8-Mediated Activation of NOX4 Signalling Restores Angiogenic Function of Endothelial Colony-Forming Cells in Experimental Hypoxia; Authors - Shun Hay Pun