Anticorps Polyclonal de lapin anti-VLDLR

• Phare
• Validé par KD/KO

VLDLR Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (1)

N° de cat : 19493-1-AP

Synonymes

VLDL-R, VLDL receptor, Very low-density lipoprotein receptor

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Galerie de données de validation

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  WB analysis using 19493-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19493-1-AP (VLDLR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 19493-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VLDLR antibody (19493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules MCF-7,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

19493-1-AP cible VLDLR dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: very low density lipoprotein receptor
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 96 kDa
• Numéro d’acquisition GenBank: NM_003383
• Symbole du gène: VLDLR
• Identification du gène (NCBI): 7436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VLDLR binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. VLDLR binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation (cytoplasmic domain of low-density lipoprotein receptor). For glycosylated, the MW of VLDLR migrates to ~160kd.

Publications

Species	Application	Title
zebrafish	WB	Neuron; Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning.; Authors - Hilliard Austin T AT
mouse	WB	Food Funct; Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice; Authors - Rui Guo
rat	WB	Front Pharmacol; Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats.; Authors - Xue Wu
mouse	WB	PLoS One; Gut microbiota and butyrate contribute to nonalcoholic fatty liver disease in premenopause due to estrogen deficiency.; Authors - Limin Liu
mouse	WB	Front Nutr; Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol; Authors - Rui Guo
human	WB,IF	Front Oncol; VLDLR disturbs quiescence of breast cancer stem cells in a ligand-independent function; Authors - Mengying Yang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

natalia (Verified Customer) (01-31-2025)

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: organoids

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