Anticorps Polyclonal de lapin anti-VPS13C

• Phare
• Validé par KD/KO

VPS13C Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 28676-1-AP

Synonymes

BLTP5C, PARK23

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Galerie de données de validation

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  WB analysis using 28676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28676-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28676-1-AP (VPS13C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 28676-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28676-1-AP (VPS13C antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

28676-1-AP cible VPS13C dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS13C Protéine recombinante Ag29434
• Nom complet: vacuolar protein sorting 13 homolog C (S. cerevisiae)
• Poids moléculaire observé: 422 kDa
• Numéro d’acquisition GenBank: NM_001018088
• Symbole du gène: VPS13C
• Identification du gène (NCBI): 54832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Publications

Species	Application	Title
human	WB	Science; LYVAC/PDZD8 is a lysosomal vacuolator; Authors - Haoxiang Yang
mouse	WB	J Cell Biol; ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling.; Authors - William Hancock-Cerutti
human,mouse	WB,IHC,IF	iScience; Downregulation of VPS13C promotes cisplatin resistance in cervical cancer by upregulating GSTP1; Authors - Xiangyu Tan ; KD Validated
human		J Cell Biol; VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons; Authors - Leonie F Schrӧder
human	WB	Biomedicines; A Pilot Study on the Proteomics Profile of Serum Exosome-Enriched Extracellular Vesicles from Normal versus Individuals with Obesity-Related Insulin Resistance; Authors - Viswanathan Saraswathi
human	WB	Contact (Thousand Oaks); VPS13A and VPS13C Influence Lipid Droplet Abundance; Authors - Shuliang Chen ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (01-25-2021)

HEK293T lysates (10ug) ran on 4-12% gradient gel prior to transfer to nitrocellulose membrane. Membrane incubated overnight at 4 degrees with antibody (1:250). Goat anti-rabbit HRP secondary antibody (1:10,000) incubated with membrane for 1 hour at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

VPS13C Antibody Western Blot validation (1:250 dilution) in HEK293T (Cat no:28676-1-AP)

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