Anticorps Polyclonal de lapin anti-VPS18

VPS18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 10901-1-AP

Synonymes

Vacuolar protein sorting-associated protein 18 homolog, KIAA1475, hVPS18

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Galerie de données de validation

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  WB analysis of mouse brain using 10901-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10901-1-AP (VPS18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10901-1-AP

  IP result of anti-VPS18 (IP:10901-1-AP, 4ug; Detection:10901-1-AP 1:300) with HeLa cells lysate 920ug.

• 

  IHC staining of human lung cancer using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10901-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 10901-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS18 antibody (10901-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral de souris, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

10901-1-AP cible VPS18 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS18 Protéine recombinante Ag1140
• Nom complet: vacuolar protein sorting 18 homolog (S. cerevisiae)
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC001513
• Symbole du gène: VPS18
• Identification du gène (NCBI): 57617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Publications

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.; Authors - Qiuhong Shen
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
human	WB	Int J Mol Sci; Polymeric Polylactic Acid-Glycolic Acid-Based Nanoparticles Deliver Nintedanib Across the Blood-Brain Barrier to Inhibit Glioblastoma Growth; Authors - Ying Dang
human	WB,IP	Nat Commun; Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17; Authors - Fenglei Jian
human	WB	Autophagy; BAG3 and SYNPO (synaptopodin) facilitate phospho-MAPT/Tau degradation via autophagy in neuronal processes.; Authors - Changyi Ji

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