Anticorps Polyclonal de lapin anti-VPS37A

• Phare
• Validé par KD/KO

VPS37A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 11870-1-AP

Synonymes

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

---

Galerie de données de validation

• 

  WB analysis using 11870-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11870-1-AP

  WB result of VPS37A antibody (11870-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS37A transfected MCF-7 cells.

• 

  WB analysis of human liver using 11870-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11870-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11870-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11870-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11870-1-AP (VPS37A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11870-1-AP (VPS37A antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human liver using 11870-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using VPS37A antibody (11870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu hépatique humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

11870-1-AP cible VPS37A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS37A Protéine recombinante Ag2453
• Nom complet: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC022363
• Symbole du gène: VPS37A
• Identification du gène (NCBI): 137492
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.

Publications

Species	Application	Title
human	WB,IF	Autophagy; Removal of hypersignaling endosomes by simaphagy; Authors - Simona M Migliano ; KO Validated
human	WB	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
human	WB	Structure; The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.; Authors - Monica Agromayor
human	WB,IHC	Cell Death Dis; HCRP-1 regulates EGFR-AKT-BIM-mediated anoikis resistance and serves as a prognostic marker in human colon cancer.; Authors - Feifei Chen ; KD Validated
human	WB	J Cell Sci; The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex.; Authors - Lydia Wunderley
human	WB	J Med Genet; A founder mutation in Vps37A causes autosomal recessive complex hereditary spastic paraparesis.; Authors - Zivony-Elboum Yifat Y