Anticorps Polyclonal de lapin anti-VPS39

• Phare
• Validé par KD/KO

VPS39 Polyclonal Antibody for IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16219-1-AP

Synonymes

hVam6p, KIAA0770, TLP, VAM6, Vam6/Vps39 like protein, VPS39

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Galerie de données de validation

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  IP experiment of mouse brain using 16219-1-AP

  IP result of anti-VPS39 (IP:16219-1-AP, 4ug; Detection:16219-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human small intestine using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

16219-1-AP cible VPS39 dans les applications de IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS39 Protéine recombinante Ag9267
• Nom complet: vacuolar protein sorting 39 homolog (S. cerevisiae)
• Masse moléculaire calculée: 886 aa, 102 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC015817
• Symbole du gène: VPS39
• Identification du gène (NCBI): 23339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway.

Publications

Species	Application	Title
human		Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
human		Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human		Autophagy; Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α; Authors - Qinyu Li
human	IF	Dev Cell; ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress; Authors - Di Chen ; KD Validated
human		Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht

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