Anticorps Polyclonal de lapin anti-VPS4A

• Phare
• Validé par KD/KO

VPS4A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 14272-1-AP

Synonymes

EC:3.6.4.6, hVPS4, Protein SKD2, SKD1, SKD2

---

Galerie de données de validation

• 

  WB analysis using 14272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 14272-1-AP

  WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells.

• 

  WB analysis using 14272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 14272-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14272-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 14272-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 14272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14272-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules U-937, cellules HepG2, cellules SMMC-7721, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

14272-1-AP cible VPS4A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS4A Protéine recombinante Ag5592
• Nom complet: vacuolar protein sorting 4 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 48-55 kDa
• Numéro d’acquisition GenBank: BC047932
• Symbole du gène: VPS4A
• Identification du gène (NCBI): 27183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vacuolar Protein Sorting 4A (VPS4A) is one of two human homologs of the yeast protein Vps4 and member of the AAA protein family (ATPases associated with diverse cellular activities).

Publications

Species	Application	Title
mouse	WB	Life Sci; Sex-specific molecular hallmarks point to increased atherogenesis susceptibility in male senescence-accelerated mice; Authors - Ainara González-Moro
canine	WB	Parasitol Res; Microautophagy upregulation in cutaneous lymph nodes of dogs naturally infected by Leishmania infantum.; Authors - Francesca De Falco
human	WB	Theranostics; Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy; Authors - Kunjian Lei
mouse	WB,IF	Mol Cell; VPS4A is the selective receptor for lipophagy in mice and humans; Authors - Debajyoti Das
human	WB	J Pharmacol Exp Ther; DBeQ derivative targets vacuolar protein sorting 4 functions in cancer cells and suppresses tumor growth in mice; Authors - Kevin A Fundora

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Nuo (Verified Customer) (11-07-2024)

This antibody can detect endogenous VPS4A with a MW of around 50 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

{{ptg:RelatedPrimaryAntibodies}}