Anticorps Polyclonal de lapin anti-VPS4A/B

• Phare
• Validé par KD/KO

VPS4A/B Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 17673-1-AP

Synonymes

VPS4B, Cell migration-inducing gene 1 protein, EC:3.6.4.6, MIG1, Protein SKD1

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Galerie de données de validation

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  WB analysis using 17673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 17673-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17673-1-AP

  IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug.

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  FC experiment of A431 using 17673-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules NIH/3T3, tissu cutané de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

17673-1-AP cible VPS4A/B dans les applications de WB, IF, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS4A/B Protéine recombinante Ag11610
• Nom complet: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Masse moléculaire calculée: 444 aa, 49 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC039574
• Symbole du gène: VPS4B
• Identification du gène (NCBI): 9525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.

Publications

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
rat	WB,IF	EMBO J; TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.; Authors - Benjamin M Schwenk
		J Cell Biol; ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.; Authors - Jorge Larios
human	WB	J Cell Sci; His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation; Authors - Gabrielle Parkinson
human	WB	Viruses; Characterization of Bovine Foamy Virus Gag Late Assembly Domain Motifs and Their Role in Recruiting ESCRT for Budding.; Authors - Zhaohuan Wang

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