Anticorps Polyclonal de lapin anti-VTA1

VTA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 15786-1-AP

Synonymes

C6orf55, Dopamine-responsive gene 1 protein, DRG 1, DRG1, DRG-1

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Galerie de données de validation

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  WB analysis using 15786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15786-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15786-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15786-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human urine using 15786-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15786-1-AP

  IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:10000) with HEK-293 cells lysate 1440 ug.

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  IHC staining of mouse stomach using 15786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15786-1-AP (VTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 15786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15786-1-AP (VTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15786-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HepG2, cellules HSC-T6, cellules K-562, cellules SMMC-7721, échantillon d'urine humaine, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu d'estomac de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15786-1-AP cible VTA1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VTA1 Protéine recombinante Ag8485
• Nom complet: Vps20-associated 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 307 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC006989
• Symbole du gène: VTA1
• Identification du gène (NCBI): 51534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomic analyses uncover a new function and mode of action for mDia2.; Authors - Tadamoto Isogai

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