Anticorps Polyclonal de lapin anti-B7‑H4

B7‑H4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12080-1-AP

Synonymes

B7 H4, B7h.5, B7H4, B7S1, B7X, PRO1291, Protein B7S1, RP11 229A19.4, VCTN1, VTCN1

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Galerie de données de validation

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  WB analysis of mouse liver using 12080-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 12080-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12080-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 12080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 12080-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 12080-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 12080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A549, cellules HeLa, tissu hépatique de rat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below

Informations sur le produit

12080-1-AP cible B7‑H4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B7‑H4 Protéine recombinante Ag2712
• Nom complet: V-set domain containing T cell activation inhibitor 1
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC065717
• Symbole du gène: VTCN1
• Identification du gène (NCBI): 79679
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

Publications

Species	Application	Title
human	IHC	Oncogene; B7-H4 is increased in lung adenocarcinoma harboring EGFR-activating mutations and contributes to immunosuppression.; Authors - Youwei Lu
human	WB	J Virol; RNASeq analysis of differentiated keratinocytes reveals a massive response to late events during human papillomavirus type 16 infection, including loss of epithelial barrier function.; Authors - T Klymenko
human	WB,IHC	Cancer Lett; Insulin-like growth factor-1 receptor induces immunosuppression in lung cancer by upregulating B7-H4 expression through the MEK/ERK signaling pathway.; Authors - Zhiming Zhao
mouse	WB,IHC	Dig Dis Sci; Dysregulated B7H4/JAK2/STAT3 Pathway Involves in Hypertriglyceridemia Acute Pancreatitis and Is Attenuated by Baicalin.; Authors - Jie Yang
human	WB	Oncol Lett; Overexpression of B7-H4 promotes renal cell carcinoma progression by recruiting tumor-associated neutrophils via upregulation of CXCL8.; Authors - Anqi Li
human	WB	J Oncol; Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.; Authors - Qiu-Ting Li