Anticorps Polyclonal de lapin anti-VWF, VWFpp

VWF, VWFpp Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(119)

Avis (1)

N° de cat : 11778-1-AP

Synonymes

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF, VWF, VWFpp

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Galerie de données de validation

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  WB analysis using 11778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11778-1-AP (VWF, VWFpp antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 11778-1-AP

  IP result of anti-VWF, VWFpp (IP:11778-1-AP, 4ug; Detection:11778-1-AP 1:300) with mouse spleen tissue lysate 5200ug.

• 

  IP experiment of mouse spleen using 11778-1-AP

  IP result of anti-VWF, VWFpp (IP:11778-1-AP, 4ug; Detection:11778-1-AP 1:300) with mouse spleen tissue lysate 5200ug.

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules Jurkat, tissu placentaire humain, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 37 publications below

Informations sur le produit

11778-1-AP cible VWF, VWFpp dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VWF, VWFpp Protéine recombinante Ag2386
• Nom complet: von Willebrand factor
• Masse moléculaire calculée: 309 kDa
• Poids moléculaire observé: 309-320 kDa, 75-100 kDa
• Numéro d’acquisition GenBank: .
• Symbole du gène: VWF
• Identification du gène (NCBI): 7450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Von Willebrand factor (vWF) is a large multimeric glycoprotein that is crucial to the hemostasis process. It supports platelet adhesion and carries factor VIII (FVIII). Deficiency of vWF results in von Willebrand disease (vWD), a common inherited bleeding disorder. The pre-pro-vWF is comprised of 2,813 amino acids (aa) which encompass a 22-aa signal peptide, a 741-aa large propeptide (vWF propeptide, vWFpp, also called von Willebrand antigen 2) and 2,050 aa making up the mature vWF. The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. This antibody raised against the N-terminal region of pre-pro-vWF recognizes von Willebrand antigen 2 (75-100 kDa) and pro-vWF (309-320 kDa). (PMID: 9759493; 12067899; 22452980; 8874191)

Publications

Species	Application	Title
mouse	WB	Cell Metab; Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction; Authors - Yuki Tatekoshi
mouse		J Thromb Haemost; Knock-in mice bearing constitutively active αIIb(R990W) mutation develop macrothrombocytopenia with severe platelet dysfunction.; Authors - Keigo Akuta
mouse		Circulation; Hyperglycemia inhibits cardiac stem cell-mediated cardiac repair and angiogenic capacity.; Authors - André S D Molgat
pig		Circulation; Intramyocardial peptide nanofiber injection improves postinfarction ventricular remodeling and efficacy of bone marrow cell therapy in pigs.; Authors - Lin Yi-Dong YD
mouse		Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
rat		Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kim-Anh (Verified Customer) (09-03-2019)

Good primary antibody, I used for IF test on HUVECs

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: huvecs

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