Anticorps Polyclonal de lapin anti-VEGFA

VEGFA Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 26157-1-AP

Synonymes

L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A, Vascular endothelial growth factor A, long form

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Galerie de données de validation

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  WB analysis of mouse brain using 26157-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26157-1-AP (Vegfa Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 26157-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26157-1-AP (Vegfa antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse lung using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26157-1-AP (VEGFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

26157-1-AP cible VEGFA dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VEGFA Protéine recombinante Ag24084
• Nom complet: vascular endothelial growth factor A
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_001287056
• Symbole du gène: Vegfa
• Identification du gène (NCBI): 22339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Publications

Species	Application	Title
mouse	WB	Carbohydr Polym; Carboxymethyl chitosan and sodium alginate oxide pH-sensitive dual-release hydrogel for diabetes wound healing: The combination of astilbin liposomes and diclofenac sodium; Authors - Xiaoyu Wu
rat	WB	Respir Res; The role of RNA m6A methylation in the regulation of postnatal hypoxia-induced pulmonary hypertension.; Authors - Shanshan Xu
human	WB	Aging (Albany NY); Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice.; Authors - Dongdong Huang
mouse	WB	Cell Biol Int; Involvement of HIF1 stabilization and VEGF signaling modulated by Grx-1 in murine model of bronchopulmonary dysplasia; Authors - Wenli Han
human	WB	Cancer Biol Ther; RSK4 promotes the metastasis of clear cell renal cell carcinoma by activating RUNX1-mediated angiogenesis; Authors - Jing Ma
rat	WB	Neuroscience; Electroacupuncture ameliorates cognitive impairment and white matter injury in vascular dementia rats via activating HIF-1α/VEGF/VEGFR2 pathway; Authors - Yihan Yang

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