Anticorps Recombinant de lapin anti-Vimentin

• Phare
• Validé par KD/KO

Vimentin Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

6K21

N° de cat : 80232-1-RR

Synonymes

VIM, 6K21

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Galerie de données de validation

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  WB analysis using 80232-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 80232-1-RR

  WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells.

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  WB analysis using 80232-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human melanoma tissue using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human melanoma slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​

• 

  IHC staining of human melanoma tissue using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human melanoma tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​

• 

  IHC staining of human breast cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse breast cancer using 80232-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 80232-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules 4T1, cellules A549, cellules HeLa, cellules HSC-T6, cellules NIH/3T3, cellules ROS1728, cellules U-251, cellules U2OS
• Résultats positifs en IHC-Autostainer: human melanoma tissue,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: mouse breast cancer,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:250-1:1000
• Immunohistochimie (IHC): IHC : 1:1250-1:5000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

80232-1-RR cible Vimentin dans les applications de WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Vimentin Protéine recombinante Ag0489
• Nom complet: vimentin
• Masse moléculaire calculée: 466 aa, 54 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC000163
• Symbole du gène: VIM
• Identification du gène (NCBI): 7431
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Publications

Species	Application	Title
human	WB	BMC Cancer; Comprehensive analysis of scRNA-seq and bulk RNA-seq data via machine learning and bioinformatics reveals the role of lysine metabolism-related genes in gastric carcinogenesis; Authors - Yongfu Shao
human	WB	J Exp Clin Cancer Res; A novel polypeptide encoded by circSPIRE1 promotes prostate cancer proliferation and migration by restraining the ubiquitin-dependent degradation of LRP5; Authors - Jintao Hu
human	WB	Front Immunol; The miR-941/FOXN4/TGF-β feedback loop induces N2 polarization of neutrophils and enhances tumor progression of lung adenocarcinoma; Authors - Xiaojing Zhang
human	WB,IF	J Neurooncol; The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells; Authors - Hui Xu
human	WB	Mol Cancer Res; Ubiquitin ligase TRIM22 inhibits ovarian cancer malignancy via TCF4 degradation; Authors - Tao Tao

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