Anticorps Polyclonal de lapin anti-WASP

• Phare
• Validé par KD/KO

WASP Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 10987-1-AP

Synonymes

IMD2, THC, THC1, WAS, WASP

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Galerie de données de validation

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  WB analysis of Raji using 10987-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10987-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10987-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10987-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10987-1-AP (WASP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 10987-1-AP

  IP Result of anti-WASP (IP:10987-1-AP, 3ug; Detection:10987-1-AP 1:500) with Raji cells lysate 3500ug.

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  IP experiment of rat spleen using 10987-1-AP

  IP result of anti-WASP (IP:10987-1-AP, 4ug; Detection:10987-1-AP 1:500) with rat spleen tissue lysate 4000 ug.

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  IHC staining of human spleen using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 10987-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10987-1-AP (WASP antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HL-60, cellules K-562, tissu splénique de souris
• Résultats positifs en IP: cellules Raji, tissu splénique de rat
• Résultats positifs en IHC: tissu splénique humain, tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

10987-1-AP cible WASP dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WASP Protéine recombinante Ag1444
• Nom complet: Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 55-62 kDa
• Numéro d’acquisition GenBank: BC012738
• Symbole du gène: WAS
• Identification du gène (NCBI): 7454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.

Publications

Species	Application	Title
mouse	WB	J Allergy Clin Immunol; Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.; Authors - Yukai Jing
human		Nat Commun; WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage.; Authors - Seong-Su Han
human	WB	J Exp Clin Cancer Res; miR-182 suppresses invadopodia formation and metastasis in non-small cell lung cancer by targeting cortactin gene.; Authors - Yongwen Li ; KD Validated
human	WB	J Exp Clin Cancer Res; CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.; Authors - Dong-Fang Meng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Seong-Su (Verified Customer) (09-17-2020)

I checked nine WASp Abs from 4 different companies for ELISA and EMSA super-shift. Among them this proteintech WASp Ab showed wonderful result for ELISA as well as was the only Ab that showed shifted bands of WASp binding complex.My colleague used this Ab for western and got a beautiful result.

• Applications: Western Blot, Other
• Primary Antibody Dilution: ELISA: 1/100 dilution, EMSA super-shift: no dilution
• Cell Tissue Type: immortalized T or B cells / B lymphoma cell lines / Jurkat

FH

Nisreen (Verified Customer) (11-07-2017)

The antibody are clean and nice.

• Applications: Western Blot