Anticorps Polyclonal de lapin anti-WDFY3/ALFY

• Phare
• Validé par KD/KO

WDFY3/ALFY Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 55009-1-AP

Synonymes

WDFY3, WDFY3 / ALFY, Alfy, Autophagy linked FYVE protein, Autophagy-linked FYVE protein

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Galerie de données de validation

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  IHC staining of rat brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55009-1-AP (WDFY3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55009-1-AP (WDFY3 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

55009-1-AP cible WDFY3/ALFY dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: WD repeat and FYVE domain containing 3
• Masse moléculaire calculée: 395 kDa
• Numéro d’acquisition GenBank: NM_014991
• Symbole du gène: WDFY3
• Identification du gène (NCBI): 23001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WDFY3, also named as KIAA0993 and Alfy, is a protein which contains WD repeats and an FYVE domain. Multiple alternatively spliced transcript variants have been found for WDFY3, but the full-length nature of some variants has not been defined. The antibody is specific to WDFY3.The band 50kd in WB is a splicing form of WDFY3.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Cardiovasc Res; WD40 repeat and FYVE domain containing 3 (Wdfy3) is essential for cardiac development.; Authors - Shasha Zhang ; KO Validated
mouse	IHC,IF	In Vitro Cell Dev Biol Anim; Autophagy-linked FYVE protein (Alfy) promotes autophagic removal of misfolded proteins involved in amyotrophic lateral sclerosis (ALS).; Authors - Huihui Han

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