Anticorps Polyclonal de lapin anti-WDR1

• Phare
• Validé par KD/KO

WDR1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 13676-1-AP

Synonymes

WD repeat containing protein 1, NORI-1, NORI 1, AIP1, Actin-interacting protein 1

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Galerie de données de validation

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  WB analysis using 13676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13676-1-AP

  WB result of WDR1 antibody (13676-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR1 transfected HEK-293 cells.

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  WB analysis of rat skeletal muscle using 13676-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13676-1-AP (WDR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 13676-1-AP

  IP result of anti-WDR1 (IP:13676-1-AP, 3ug; Detection:13676-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human testis using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13676-1-AP (WDR1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 13676-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13676-1-AP (WDR1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules U-87, muscle squelettique de souris, tissu de muscle squelettique de rat
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 1 publications below

Informations sur le produit

13676-1-AP cible WDR1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR1 Protéine recombinante Ag4670
• Nom complet: WD repeat domain 1
• Masse moléculaire calculée: 606 aa, 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC030541
• Symbole du gène: WDR1
• Identification du gène (NCBI): 9948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WDR1, which belongs to the WD repeat AIP1 family, also known as AIP1, is a 606 amino acid protein that localizes to the cytoskeleton. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WDR1 induces the disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PMID:15629458, 27557945, 29751004). WDR1 mutations may impair the ability of WDR1 to regulate cofilin-mediated actin depolymerization, and are associated with periodic fever, immunodeficiency, and thrombocytopenia syndrome (PMID: 27557945,27994071,29751004).

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; AIP1-mediated actin disassembly is required for postnatal germ cell migration and spermatogonial stem cell niche establishment.; Authors - J Xu
human	WB	J Biol Chem; Trp-Asp (WD) repeat domain 1 is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation.; Authors - Yi Xiao
mouse	WB	Am J Pathol; A Cardiomyocyte-Specific Wdr1 Knockout Demonstrates Essential Functional Roles for Actin Disassembly during Myocardial Growth and Maintenance In Mice.; Authors - Baiyin Yuan ; KO Validated
human,mouse	WB	Mol Cells; WD Repeat Domain 1 Deficiency Inhibits Neointima Formation in Mice Carotid Artery by Modulation of Smooth Muscle Cell Migration and Proliferation.; Authors - JiSheng Hu ; KO Validated
human	WB	Int J Biol Sci; WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics.; Authors - Baiyin Yuan ; KD Validated
mouse	WB	Dev Biol; WDR1-regulated actin dynamics is required for outflow tract and right ventricle development.; Authors - Jisheng Hu ; KO Validated

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