Anticorps Polyclonal de lapin anti-WDR24

• Phare
• Validé par KD/KO

WDR24 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 20778-1-AP

Synonymes

C16orf21, JFP7, WD repeat domain 24, WDR24

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Galerie de données de validation

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  WB analysis of HEK-293 using 20778-1-AP

  WB result of WDR24 antibody (20778-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.

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  WB analysis using 20778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20778-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20778-1-AP

  IP result of anti-WDR24(IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:3000) with HeLa cells lysate 1560 ug.

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  IHC staining of human colon cancer using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 20778-1-AP

  Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20778-1-AP

  Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IF: See 2 publications below
• IP: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

20778-1-AP cible WDR24 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WDR24 Protéine recombinante Ag14774
• Nom complet: WD repeat domain 24
• Masse moléculaire calculée: 920 aa, 102 kDa
• Poids moléculaire observé: 102 kDa, 88 kDa
• Numéro d’acquisition GenBank: BC008025
• Symbole du gène: WDR24
• Identification du gène (NCBI): 84219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

Publications

Species	Application	Title
	WB,IP	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
human	IP,WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long
human	WB,IP	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
mouse	WB,CoIP	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
human	WB	Nature; SZT2 dictates GATOR control of mTORC1 signalling.; Authors - Min Peng ; KO Validated
human	WB	Nat Commun; Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation; Authors - Xing Chen

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