Anticorps Polyclonal de lapin anti-WEE1

WEE1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 29474-1-AP

Synonymes

DKFZp686I18166, FLJ16446, WEE1, WEE1 homolog (S. pombe), Wee1 like protein kinase, WEE1A, Wee1A kinase, WEE1hu

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Galerie de données de validation

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  WB analysis using 29474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29474-1-AP (WEE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu cardiaque de souris, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

29474-1-AP cible WEE1 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WEE1 Protéine recombinante Ag30194
• Nom complet: WEE1 homolog (S. pombe)
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa,110 kDa
• Numéro d’acquisition GenBank: BC051831
• Symbole du gène: WEE1
• Identification du gène (NCBI): 7465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WEE1 kinase is a serine-threonine kinase that regulates G2/M checkpoint transition. WEE1 triggers G2/M arrest through inhibitory phosphorylation on Tyr15 of CDK1 (Cdc2) and preventing entry into mitosis to allow DNA repair during DNA damage (PMID: 27427153).The predicted native molecular weight for Wee1 based on the intact human Wee1 cDNA sequence is 72 kDa, whereas the 95-110 kDa protein is a highly modified product (PMID: 7568188). Others have shown that Wee1 is 70-72 kDa and 49-55 kDa (PMID: 12214061).

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; DIS3L2 ribonuclease degrades terminal-uridylated RNA to ensure oocyte maturation and female fertility; Authors - Di Wu
human	WB	J Cell Mol Med; Venetoclax enhances DNA damage induced by XPO1 inhibitors: A novel mechanism underlying the synergistic antileukaemic effect in acute myeloid leukaemia.; Authors - Hanxi Yu
mouse	WB	Reprod Toxicol; High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.; Authors - Yi-Ming Ji
human	WB	Anal Cell Pathol (Amst); miR-138-5p Inhibits the Growth and Invasion of Glioma Cells by Regulating WEE1.; Authors - Jianwu Gong
human,mouse	WB,CoIP	J Pharm Pharmacol; GL-V9 synergizes with oxaliplatin of colorectal cancer via Wee1 degradation mediated by HSP90 inhibition; Authors - Hongyu Chen
mouse	WB	Ren Fail; Hyperactivation of p53 contributes to mitotic catastrophe in podocytes through regulation of the Wee1/CDK1/cyclin B1 axis; Authors - Jie Feng

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