Anticorps Monoclonal anti-WFS1

WFS1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

4C2B5

N° de cat : 67291-1-Ig

Synonymes

4C2B5, WFRS, WFS, WOLFRAMIN

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Galerie de données de validation

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  WB analysis using 67291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A172 using 67291-1-Ig

  A172 cells were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67291-1-Ig (WFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules HepG2, cellules HT-29, cellules Neuro-2a, cellules PC-12, cellules SH-SY5Y, cellules SW480, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67291-1-Ig cible WFS1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WFS1 Protéine recombinante Ag25735
• Nom complet: Wolfram syndrome 1 (wolframin)
• Masse moléculaire calculée: 890 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC030130
• Symbole du gène: WFS1
• Identification du gène (NCBI): 7466
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Wolfram syndrome protein (WFS1), also called wolframin, is a transmembrane protein, which is located primarily in the endoplasmic reticulum and its expression is induced in response to ER stress, partially through transcriptional activation. ER localization suggests that WFS1 protein has physiological functions in membrane trafficking, secretion, processing and/or regulation of ER calcium homeostasis. It is ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations of the WFS1 gene are responsible for two hereditary diseases, autosomal recessive Wolfram syndrome and autosomal dominant low frequency sensorineural hearing loss.

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