Anticorps Polyclonal de lapin anti-NSD3

• Phare
• Validé par KD/KO

NSD3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 11345-1-AP

Synonymes

WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3

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Galerie de données de validation

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  WB analysis of HeLa using 11345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 11345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11345-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11345-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11345-1-AP (NSD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11345-1-AP

  IP result of anti-NSD3 (IP:11345-1-AP, 4ug; Detection:11345-1-AP 1:600) with HeLa cells lysate 1085 ug.

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  IHC staining of human lung squamous cell carcinoma using 11345-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 11345-1-AP (NSD3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 11345-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NSD3 antibody (11345-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of NIH/3T3 using 11345-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug NSD3 Polyclonal antibody (11345-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: human lung squamous cell carcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

11345-1-AP cible NSD3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NSD3 Protéine recombinante Ag1894
• Nom complet: Wolf-Hirschhorn syndrome candidate 1-like 1
• Masse moléculaire calculée: 1437 aa, 162 kDa
• Poids moléculaire observé: 160-170 kDa, 68-72 kDa
• Numéro d’acquisition GenBank: BC012059
• Symbole du gène: WHSC1L1
• Identification du gène (NCBI): 54904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

Publications

Species	Application	Title
human	WB	Sci Adv; Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair; Authors - Liu Liu
human	IHC	Cancer Res; NSD3-Induced Methylation of H3K36 Activates NOTCH Signaling to Drive Breast Tumor Initiation and Metastatic Progression.; Authors - Ga-Young Jeong
human	WB	Cancer Res; Transforming properties of 8p11-12 amplified genes in human breast cancer.; Authors - Yang Zeng-Quan ZQ ; KD Validated
		Mol Oncol; Amplification of WHSC1L1 regulates expression and estrogen-independent activation of ERα in SUM-44 breast cancer cells and is associated with ERα over-expression in breast cancer.; Authors - Jonathan C Irish
		Sci Rep; NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation.; Authors - Verónica García-Carpizo
human	WB,IF	Sci Rep; WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer.; Authors - Vassiliki Saloura ; KD Validated

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