Anticorps Polyclonal de lapin anti-WIPI1

• Phare
• Validé par KD/KO

WIPI1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 25204-1-AP

Synonymes

WIPI-1, WIPI 49 kDa, WIPI 1, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog

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Galerie de données de validation

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  WB analysis of SW 1990 using 25204-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 25204-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25204-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25204-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25204-1-AP (WIPI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 25204-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25204-1-AP (WIPI1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SW-1990, cellules HEK-293, cellules NIH/3T3
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

25204-1-AP cible WIPI1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WIPI1 Protéine recombinante Ag18461
• Nom complet: WD repeat domain, phosphoinositide interacting 1
• Masse moléculaire calculée: 446 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC039867
• Symbole du gène: WIPI1
• Identification du gène (NCBI): 55062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WIPI1, or WD repeat domain, phosphoinositide-interacting protein 1, is a member of the WD40 repeat family of proteins, which are key components of many essential biological functions. WIPI1 is involved in the regulation of autophagy, a cellular process critical for maintaining cellular integrity and recycling intracellular proteins, lipids, and organelles. WIPI1 is characterized by its 7-bladed β-propeller structure and contains a conserved motif for interaction with phospholipids, specifically binding to phosphoinositides. This interaction is crucial for its function in autophagy, where it acts as an effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI1 and WIPI2 are among the first proteins to be recruited during autophagy, with WIPI2 interacting with components of the ULK1/2 complex and PtdIns3P, tethering the phagophore to the endoplasmic reticulum (ER). WIPI1 supports the function of WIPI2 in recruiting the ATG16L complex, which is essential for LC3 lipidation and autophagosome formation.

Publications

Species	Application	Title
human	WB,IP	Oral Oncol; WIPI-1 inhibits metastasis and tumour growth via the WIPI-1-TRIM21 axis and MYC regulation in nasopharyngeal carcinoma.; Authors - Yin Zhao ; KD Validated
mouse	WB	Phytomedicine; Intestinal 8 gingerol attenuates TBI-induced neuroinflammation by inhibiting microglia NLRP3 inflammasome activation in a PINK1/Parkin-dependent manner; Authors - Xuheng Tang

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