Anticorps Polyclonal de lapin anti-WISP-1/CCN4

• Phare
• Validé par KD/KO

WISP-1/CCN4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 18166-1-AP

Synonymes

WISP1, CCN4, WISP-1, Wnt-1-induced secreted protein, WNT1-inducible-signaling pathway protein 1

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Galerie de données de validation

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  WB analysis using 18166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18166-1-AP (WISP-1/CCN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18166-1-AP (WISP-1/CCN4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 18166-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18166-1-AP (WISP-1/CCN4 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du poumon humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18166-1-AP cible WISP-1/CCN4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WISP-1/CCN4 Protéine recombinante Ag12657
• Nom complet: WNT1 inducible signaling pathway protein 1
• Masse moléculaire calculée: 367 aa, 40 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC074841
• Symbole du gène: WISP1
• Identification du gène (NCBI): 8840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WISP1( Wnt-induced secreted protein 1), also known as CCN4, is a secreted, matricellular protein allocated to the CCN protein family. The CCN protein family consists of six, modular structured, secreted proteins ( Cyr61, CTGF, Nov, WISP1, WISP2, WISP3 ). WISP1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. WISP1 is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. Its expression is associated with proliferation, cytoprotection, as well as extracellular matrix production. 18166-1-AP antibody detects the 40-45 kDa protein in SDS-PAGE.

Publications

Species	Application	Title
	WB	Am J Respir Crit Care Med; Critical Genomic Networks and Vasoreactive Variants in Idiopathic Pulmonary Arterial Hypertension.; Authors - Anna R Hemnes
human	WB,IF	Exp Mol Med; WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling.; Authors - Chao Sun ; KD Validated
human	WB	Food Chem Toxicol; Quinocetone induces mitochondrial apoptosis in HepG2 cells through ROS-dependent promotion of VDAC1 oligomerization and suppression of Wnt1/β-catenin signaling pathway.; Authors - Xiayun Yang
human	WB,IHC	Am J Transl Res; High expression of WISP-1 correlates with poor prognosis in pancreatic ductal adenocarcinoma.; Authors - Jian-Yu Yang
		Int J Oncol; Identification of WISP1 as a novel oncogene in glioblastoma.; Authors - Di Jing
human	WB	Oncol Lett; MMP12 is a potential therapeutic target for Adamantinomatous craniopharyngioma: Conclusions from bioinformatics analysis and in vitro experiments.; Authors - Minde Li

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