Anticorps Monoclonal anti-WNK1

WNK1 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1B6A10

N° de cat : 68149-1-Ig

Synonymes

1B6A10, EC:2.7.11.1, HSAN2, HSN2, KDP

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Galerie de données de validation

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  WB analysis using 68149-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68149-1-Ig (WNK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293T using 68149-1-Ig

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human WNK1 (68149-1-Ig, Clone:1B6A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68149-1-Ig cible WNK1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WNK1 Protéine recombinante Ag28044
• Nom complet: WNK lysine deficient protein kinase 1
• Masse moléculaire calculée: 251 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC021121
• Symbole du gène: WNK1
• Identification du gène (NCBI): 65125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WNK kinase is a unique serine-threonine kinase in which the lysine (K) residue in the catalytic domain, that is found in the majority of kinases is not conserved. In mammals, there are four WNK kinase family members (WNK1-4). WNK1 has 6 isoforms, such as long form WNK1 (L-WNK1, 250 kDa, strong expression in dorsal root ganglia and spinal cord) ; kidney-specific isoform (KS-WNK1, 207 kDa, lacks a kinase domain).KS-WNK1 cannot phosphorylate substrates and instead interacts with and modulates the actions of other WNKs via a carboxyl terminal HQ domain.

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