Anticorps Polyclonal de lapin anti-WNT1

• Phare
• Validé par KD/KO

WNT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(52)

N° de cat : 27935-1-AP

Synonymes

Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, Proto oncogene Wnt 1, Proto oncogene Int 1 homolog, INT1

---

Galerie de données de validation

• 

  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 27935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 27935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT1 antibody (27935-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of NIH/3T3 using 27935-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  FC experiment of HeLa using 27935-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human WNT1 (27935-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules 3T3-L1, cellules HepG2, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules HeLa
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 52 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

27935-1-AP cible WNT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT1 Protéine recombinante Ag27380
• Nom complet: wingless-type MMTV integration site family, member 1
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC074799
• Symbole du gène: WNT1
• Identification du gène (NCBI): 7471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Bioengineered; MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1); Authors - Guang-Lin Song
human	WB	Neural Regen Res; Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke; Authors - Jianfeng Wang
human	WB	Aging (Albany NY); Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway.; Authors - Liansheng Sha
mouse	WB	J Ethnopharmacol; Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice; Authors - Yin OuYang
mouse	WB	Microb Biotechnol; Combination of an engineered Lactococcus lactis expressing CXCL12 with light-emitting diode yellow light as a treatment for scalded skin in mice.; Authors - Xiaoxiao Zhao
mouse	WB	Mol Neurobiol; GPR30 Inhibits Neuronal Apoptosis After Subarachnoid Hemorrhage by Activating the Wnt/β-Catenin Pathway in a m6A-dependent Manner; Authors - Jun Peng

{{ptg:RelatedPrimaryAntibodies}}