Anticorps Polyclonal de lapin anti-WNT10A

WNT10A Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 26238-1-AP

Synonymes

WNT10, Protein Wnt-10a, Protein Wnt 10a

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Galerie de données de validation

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus cancer using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 26238-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of NIH/3T3 using 26238-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 26238-1-AP (WNT10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 26238-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26238-1-AP (WNT10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules HeLa

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

26238-1-AP cible WNT10A dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT10A Protéine recombinante Ag23963
• Nom complet: wingless-type MMTV integration site family, member 10A
• Masse moléculaire calculée: 417 aa, 46 kDa
• Numéro d’acquisition GenBank: BC052234
• Symbole du gène: WNT10A
• Identification du gène (NCBI): 80326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WNT10A is a member of the WNT gene family, which consists of structurally related genes encoding secreted signaling molecules critical for many organogenesis processes. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines.

Publications

Species	Application	Title
human	IHC	Cancer Cell Int; Identification of the prognostic value of a 2-gene signature of the WNT gene family in UCEC using bioinformatics and real-world data.; Authors - Yuexin Hu
human	IHC	Oral Dis; Long non-coding RNAs MALAT1 and NEAT1 in non-syndromic orofacial clefts.; Authors - Errui Wang
goat	WB,IF	J Anim Sci Biotechnol; Melatonin promotes the development of the secondary hair follicles by regulating circMPP5; Authors - Xiaogao Diao
mouse	IHC	Kidney Int; Non-canonical Wnt/calcium signaling is protective against podocyte injury and glomerulosclerosis; Authors - Dong Zhou
human,mouse	WB,IHC	Cancer Biother Radiopharm; Raddeanin A Inhibits Colorectal Cancer Growth and Ameliorates Oxaliplatin Resistance Through the WNT/β-Catenin Signaling Pathway; Authors - Junguo Chen ; KD Validated
		Mol Ther Nucleic Acids; Mechanical stimuli-induced CCL2 restores adult mouse cells to regenerate hair follicles; Authors - Wang Wu

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