Anticorps Polyclonal de lapin anti-WNT6

WNT6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 24201-1-AP

Synonymes

Protein Wnt 6, WNT6

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Galerie de données de validation

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  WB analysis of SGC-7901 using 24201-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 24201-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 24201-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24201-1-AP (WNT6 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SGC-7901
• Résultats positifs en IHC: tissu cérébral de souris, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

24201-1-AP cible WNT6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WNT6 Protéine recombinante Ag20889
• Nom complet: wingless-type MMTV integration site family, member 6
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC004329
• Symbole du gène: WNT6
• Identification du gène (NCBI): 7475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WNT6 belongs to the Wnt family. It is ligand for members of the frizzled family of seven transmembrane receptors. WNT6 is likely to signal over only few cell diameters. WNT6 may be together with CAV1 to promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway. And WNT6 is mainly expressed in brain, endometrium epithelium, gastric cancer cell lines and gastric cancer tissues (PMID: 22370641).

Publications

Species	Application	Title
human,chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
human	WB	Front Oncol; YTHDF1 Regulates Tumorigenicity and Cancer Stem Cell-Like Activity in Human Colorectal Carcinoma.; Authors - Yang Bai
human	WB	Am J Physiol Cell Physiol; Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.; Authors - Qin Ju He
human	WB,IHC,IF	Gene; WNT6 participates in the occurrence and development of ovarian cancer by upregulating/activating the typical Wnt pathway and Notch1 signaling pathway; Authors - Haijuan Bao ; KD Validated
human	WB,IHC	Oncol Lett; Correlation study of DNA methylation of WNT6 gene with osteosarcoma in children.; Authors - Li Li
human	WB	Clin Transl Med; LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A; Authors - Danlei Chen