Anticorps Polyclonal de lapin anti-WTAP

• Phare
• Validé par KD/KO

WTAP Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(68)

N° de cat : 10200-1-AP

Synonymes

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, Mum2, KIAA0105, FL2D

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Galerie de données de validation

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  WB analysis of HeLa using 10200-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10200-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat cells using 10200-1-AP

  WB result of WTAP specific antibody (10200-1-AP, 1:6000) with si-control and si-WTAP transfected Jurkat cells.

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  WB analysis using 10200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10200-1-AP

  IP result of anti-WTAP (IP:10200-1-AP, 3ug; Detection:10200-1-AP 1:500) with HEK-293 cells lysate 2500ug.

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  IP experiment of Jurkat using 10200-1-AP

  IP result of anti-WTAP (IP:10200-1-AP, 4ug; Detection:10200-1-AP 1:8000) with Jurkat cells lysate 2280 ug.

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  IHC staining of human stomach using 10200-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 10200-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules L02, cellules MOLT-4
• Résultats positifs en IP: cellules Jurkat, cellules HEK-293
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 14 publications below
• WB: See 62 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 4 publications below

Informations sur le produit

10200-1-AP cible WTAP dans les applications de WB, IHC, IF, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WTAP Protéine recombinante Ag0259
• Nom complet: Wilms tumor 1 associated protein
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC000383
• Symbole du gène: WTAP
• Identification du gène (NCBI): 9589
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.

Publications

Species	Application	Title
mouse	WB	Nature; m6A RNA methylation regulates the fate of endogenous retroviruses.; Authors - Tomasz Chelmicki ; KO Validated
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu ; KD Validated
mouse	WB	Cell Metab; Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11; Authors - Bing Zhou
mouse	WB,RIP	Nat Cell Biol; The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.; Authors - Xiang-Qian Gao
human	WB	Mol Cell; Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.; Authors - Wenqi Xu ; KD Validated
mouse	WB,IF	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen

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