Anticorps Monoclonal anti-WWP1

• Phare
• Validé par KD/KO

WWP1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2G8G11

N° de cat : 67804-1-Ig

Synonymes

2G8G11, AIP5, Atrophin-1-interacting protein 5, EC:2.3.2.26, HECT-type E3 ubiquitin transferase WWP1

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Galerie de données de validation

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  WB analysis using 67804-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HaCaT using 67804-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HaCaT, cellules HeLa, cellules Jurkat, cellules LNCaP

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

67804-1-Ig cible WWP1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WWP1 Protéine recombinante Ag30171
• Nom complet: WW domain containing E3 ubiquitin protein ligase 1
• Masse moléculaire calculée: 922 aa, 105 kDa
• Poids moléculaire observé: 95-105 kDa
• Numéro d’acquisition GenBank: BC036065
• Symbole du gène: WWP1
• Identification du gène (NCBI): 11059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse,human	WB	Cancers (Basel); GluOC Induced SLC7A11 and SLC38A1 to Activate Redox Processes and Resist Ferroptosis in TNBC; Authors - Jiaojiao Xu ; KD Validated

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