Anticorps Polyclonal de lapin anti-WWP2

• Phare
• Validé par KD/KO

WWP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 12197-1-AP

Synonymes

AIP2, WW domain containing protein 2, WWP2, WWp2 like

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Galerie de données de validation

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  WB analysis of mouse liver using 12197-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12197-1-AP (WWP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12197-1-AP

  WB result of WWP2 antibody (12197-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWP2 transfected HepG2 cells.

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  WB analysis of A549 using 12197-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12197-1-AP (WWP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12197-1-AP

  IP result of anti-WWP2 (IP:12197-1-AP, 4ug; Detection:12197-1-AP 1:1000) with A549 cells lysate 800 ug.

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  IHC staining of human pancreas cancer using 12197-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12197-1-AP (WWP2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A549, cellules HepG2
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12197-1-AP cible WWP2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: WWP2 Protéine recombinante Ag2835
• Nom complet: WW domain containing E3 ubiquitin protein ligase 2
• Masse moléculaire calculée: 870 aa, 99 kDa
• Poids moléculaire observé: 110-115 kDa
• Numéro d’acquisition GenBank: BC013645
• Symbole du gène: WWP2
• Identification du gène (NCBI): 11060
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

WWP2(WW domain-containing protein 2) is also named as AIP2 and belongs to the NEDD4-like protein family. It functions as an E3 ubiquitin ligase for PTEN that plays a vital role in tumour-cell survival and is also involved in regulating transcription, embryonic stem-cell fate, cellular transport and T-cell activation processes(PMID:21532586).WWP2, which specifically interacted with ES cell-specific transcription factor Oct-4 and promoted its ubiquitination both in vitro and in vivo, is a ubiquitously expressed protein and exists in both cytoplasmic and nuclear compartments in ES cells(PMID:15047715).

Publications

Species	Application	Title
	WB,IF	Cardiovasc Diabetol; Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X; Authors - Shilong You ; KD Validated
human	WB	Cell Death Differ; Selective targeting of ubiquitination and degradation of PARP1 by E3 ubiquitin ligase WWP2 regulates isoproterenol-induced cardiac remodeling.; Authors - Naijin Zhang ; KD Validated
mouse	WB	Redox Biol; Role of WW domain E3 ubiquitin protein ligase 2 in modulating ubiquitination and Degradation of Septin4 in oxidative stress endothelial injury.; Authors - Naijin Zhang
human	WB	Br J Pharmacol; Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.; Authors - Mao-Xu Ge
mouse	WB,IHC,IF	J Dent Res; WWP2 Promotes Odontoblastic Differentiation by Monoubiquitinating KLF5.; Authors - J Fu
mouse	WB	J Cell Mol Med; WWP2 regulates SIRT1-STAT3 acetylation and phosphorylation involved in hypertensive angiopathy.; Authors - Ying Zhang