Anticorps Polyclonal de lapin anti-XBP-1U specific

• Phare
• Validé par KD/KO

XBP-1U specific Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

43

N° de cat : 25997-1-AP

Synonymes

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1s, XBP-1S, XBP1U, XBP-1U specific, XBP2

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Galerie de données de validation

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  WB analysis of rat spleen using 25997-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 25997-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25997-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25997-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 25997-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP-1U specific (25997-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 40 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

25997-1-AP cible XBP-1U specific dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XBP-1U specific Protéine recombinante Ag21714
• Nom complet: X-box binding protein 1
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000938
• Symbole du gène: XBP1
• Identification du gène (NCBI): 7494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

X-box-binding protein 1 (XBP1), also named as TREB5, is a 261 amino acid protein, which contains one bZIP domain and belongs to the bZIP family. XBP1 localizes in the nucleus and as a transcription factor is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The molecular weight of protein generated from the spliced XBP1 mRNA is 54 kDa and protein generated from unspliced Xbp1 mRNA is 33 kDa. This antibody 25997-1-AP specially recognizes isoform XBP-1U.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
mouse	WB	Hepatology; Farnesoid X receptor signaling activates the hepatic X-box binding protein 1 pathway in vitro and in mice.; Authors - Xiaoying Liu
human	IHC,IF	Sci Adv; Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.; Authors - Can Huang
mouse	WB	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
pig	WB	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming
human	WB	Oncogene; Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.; Authors - Yifei Zhu