Anticorps Polyclonal de lapin anti-XPO6

• Phare
• Validé par KD/KO

XPO6 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF

Conjugaison

Non conjugué

Publications

7

N° de cat : 11408-1-AP

Synonymes

EXP6, exportin 6, KIAA0370, Ran binding protein 20, RANBP20, XPO6

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Galerie de données de validation

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  WB analysis of HeLa using 11408-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11408-1-AP (XPO6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11408-1-AP

  IP Result of anti-XPO6 (IP:11408-1-AP, 4ug; Detection:11408-1-AP 1:300) with HeLa cells lysate 4000ug.

• 

  IHC staining of human heart using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11408-1-AP (XPO6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11408-1-AP (XPO6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human ovary using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human ovary using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human kidney using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human kidney using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human placenta using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human placenta using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human skin using 11408-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11408-1-AP (XPO6 antibody) at dilution of 1:25 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

11408-1-AP cible XPO6 dans les applications de WB, IP, IHC, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Drosophile, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: XPO6 Protéine recombinante Ag1898
• Nom complet: exportin 6
• Masse moléculaire calculée: 1125 aa, 129 kDa
• Poids moléculaire observé: 128 kDa
• Numéro d’acquisition GenBank: BC014071
• Symbole du gène: XPO6
• Identification du gène (NCBI): 23214
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human XPO6 gene encodes a Ran-binding protein 20, exportin-6, belonging to the exportin family. Exportin-6 shuttles between the nucleus and the cytoplasm and mediates the nuclear export of actin and profilin-actin complexes in somatic cells. Interaction of exportin-6 and actin is achieved in a RanGTP-dependent manner, actin is a major cytoskeletal element which is normally kept cytoplasmic by exportin-6 driven nuclear export.

Publications

Species	Application	Title
human	WB	Nat Commun; Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.; Authors - Sergi Marco
Drosophila	IF	Proc Natl Acad Sci U S A; Active maintenance of nuclear actin by importin 9 supports transcription.; Authors - Dopie Joseph J
human	WB	Cancer Res; Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer.; Authors - Ambs Stefan S
human	WB	J Virol; The XPO6 Exportin Mediates Herpes Simplex Virus 1 gM Nuclear Release Late in Infection.; Authors - Hugo Boruchowicz ; KD Validated
human	WB	RNA; An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.; Authors - Ewan K S McRae
human	WB	JCI Insight; Potent antitumor effects of cell-penetrating peptides targeting STAT3 axis.; Authors - Maryam Aftabizadeh