Anticorps Monoclonal anti-XRCC3

XRCC3 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

3A12F9

N° de cat : 67222-1-Ig

Synonymes

DNA repair protein XRCC3, XRCC3

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Galerie de données de validation

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  WB analysis using 67222-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 67222-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 67222-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 67222-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 67222-1-Ig

  IP result of anti-XRCC3 (IP:67222-1-Ig, 5ug; Detection:67222-1-Ig 1:500) with MCF-7 cells lysate 640 ug.

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  IHC staining of mouse testis using 67222-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67222-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67222-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 67222-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 67222-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299, cellules Caco-2, cellules COLO 320, cellules HeLa, cellules HT-1080, cellules HT-29, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67222-1-Ig cible XRCC3 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: XRCC3 Protéine recombinante Ag13317
• Nom complet: X-ray repair complementing defective repair in Chinese hamster cells 3
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC011725
• Symbole du gène: XRCC3
• Identification du gène (NCBI): 7517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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