Anticorps Polyclonal de lapin anti-MSY2

MSY2 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13538-1-AP

Synonymes

CONTRIN, CSDA3, DBPC, DNA binding protein C, MSY2, MSY2 homolog, Y box binding protein 2, YBX2

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Galerie de données de validation

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  WB analysis using 13538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13538-1-AP (MSY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13538-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13538-1-AP (MSY2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 13538-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13538-1-AP (MSY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 13538-1-AP

  IP Result of anti-MSY2 (IP:13538-1-AP, 4ug; Detection:13538-1-AP 1:300) with PC-3 cells lysate 1720ug.

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  IHC staining of human ovary tumor using 13538-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13538-1-AP (MSY2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 13538-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13538-1-AP (MSY2 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu d'estomac humain, tissu testiculaire humain
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

13538-1-AP cible MSY2 dans les applications de WB, IP, IHC, IF, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MSY2 Protéine recombinante Ag4465
• Nom complet: Y box binding protein 2
• Masse moléculaire calculée: 364 aa, 39 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC033800
• Symbole du gène: YBX2
• Identification du gène (NCBI): 51087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSY2, also named as CSDA3 and YBX2, belongs to the Y box family of cold shock domain proteins. MSY2 is solely expressed in male and female germ cells and necessary for fertility. MSY2 serves as a coactivator of transcription, binding to the DNA sequence CTGATTGGC/TC/TAA in the promoters of genes transcribed in germ cells, thereby linking transcription with mRNA storage/stabilization (PMID: 19597149). MSY2 has 1 isoform with the molecular mass of 38 kDa. MSY2 is essential for regulating mRNA stability in oocytes (PMID: 21613634).

Publications

Species	Application	Title
mouse	WB,IHC,chIP	J Clin Invest; Methylation in pericytes after acute injury promotes chronic kidney disease.; Authors - Yu-Hsiang Chou
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang