Anticorps Polyclonal de lapin anti-YES1-Specific

• Phare
• Validé par KD/KO

YES1-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 20243-1-AP

Synonymes

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1, YES1-Specific

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Galerie de données de validation

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  WB analysis using 20243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20243-1-AP (YES1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20243-1-AP

  WB result of YES1-Specific antibody (20243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1-Specific transfected HepG2 cells.

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  IP experiment of A549 using 20243-1-AP

  IP result of anti-YES1-Specific(IP:20243-1-AP, 4ug; Detection:20243-1-AP 1:500) with A549 cells lysate 1400 ug.

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  IHC staining of human lung cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20243-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20243-1-AP (YES1-Specific antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules DU 145, cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

20243-1-AP cible YES1-Specific dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: NM_005433
• Symbole du gène: YES1
• Identification du gène (NCBI): 7525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.

Publications

Species	Application	Title
human	IHC	Am J Respir Crit Care Med; YES1 Drives Lung Cancer Growth and Progression and Predicts Sensitivity to Dasatinib.; Authors - Irati Garmendia
human,mouse		Sci Adv; A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.; Authors - Jackson A Roberts
human	IHC	J Immunother Cancer; SRC family kinase (SFK) inhibitor dasatinib improves the antitumor activity of anti-PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferation.; Authors - Esther Redin ; KO Validated
human		Cell Death Dis; Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p.; Authors - Yujing Sun
human		J Cell Biochem; miR-17-5p up-regulates YES1 to modulate the cell cycle progression and apoptosis in ovarian cancer cell lines.; Authors - Lan Li ; KD Validated
human		iScience; DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer; Authors - Qingwen Huang