Anticorps Polyclonal de lapin anti-YPEL5

YPEL5 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 11730-1-AP

Synonymes

CGI 127, Protein yippee like 5, yippee like 5 (Drosophila), YPEL5

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Galerie de données de validation

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  WB analysis using 11730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11730-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11730-1-AP (YPEL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11730-1-AP (YPEL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

11730-1-AP cible YPEL5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YPEL5 Protéine recombinante Ag2328
• Nom complet: yippee-like 5 (Drosophila)
• Masse moléculaire calculée: 121 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC000836
• Symbole du gène: YPEL5
• Identification du gène (NCBI): 51646
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YPEL5(Protein yippee-like 5) belongs to the yippee family and is involved in a certain cell division-related function.During cell cycle, YPEL5 protein is detected at different subcellular localizations; at interphase, it is located in the nucleus and centrosome, then it changes location sequentially to spindle poles, mitotic spindle, and spindle midzone during mitosis, and finally transferrs to midbody at cytokinesis(PMID:20580816).

Publications

Species	Application	Title
zebrafish	WB,IF	Neuron; Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning.; Authors - Hilliard Austin T AT
human	WB	Mol Oncol; METTL3/YTHDF2 m6A axis accelerates colorectal carcinogenesis through epigenetically suppressing YPEL5.; Authors - Dan Zhou
human	WB	iScience; Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24; Authors - Lalit R Patel

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

The band was there on the right MW range, but it was a bit weak. It could have been better if I used 0.2 um pore-size membrane, given that YPEL5 is a small protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

YPEL5 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11730-1-AP)