Anticorps Polyclonal de lapin anti-YTHDC1

• Phare
• Validé par KD/KO

YTHDC1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 14392-1-AP

Synonymes

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

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Galerie de données de validation

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  WB analysis of HEK-293 using 14392-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse tissue using 14392-1-AP

  WB result of YTHDC1 (14392-1-AP) antibody with mouse sample.

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  WB analysis of MCF-7 using 14392-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7
• Résultats positifs en IHC: tissu cérébral de souris, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 5 publications below

Informations sur le produit

14392-1-AP cible YTHDC1 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YTHDC1 Protéine recombinante Ag5882
• Nom complet: YTH domain containing 1
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: ~100 kDa
• Numéro d’acquisition GenBank: BC053863
• Symbole du gène: YTHDC1
• Identification du gène (NCBI): 91746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).

Publications

Species	Application	Title
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
human	WB,IF	Nucleic Acids Res; N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.; Authors - Haojie Hao
human	WB	Cell Death Differ; YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect.; Authors - Yichao Hou
mouse	RIP	Dev Cell; N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.; Authors - Jie Du
human	WB,IP	Mol Ther Nucleic Acids; METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitination.; Authors - Danyang Shen ; KD Validated