Anticorps Polyclonal de lapin anti-YTHDF1

YTHDF1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 26787-1-AP

Synonymes

C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1

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Galerie de données de validation

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  WB analysis of HepG2 using 26787-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26787-1-AP (YTHDF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26787-1-AP

  IP result of anti-YTHDF1 (IP:26787-1-AP, 4ug; Detection:26787-1-AP 1:300) with HepG2 cells lysate 3200ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2,
• Résultats positifs en IP: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

26787-1-AP cible YTHDF1 dans les applications de WB, IHC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YTHDF1 Protéine recombinante Ag25347
• Nom complet: YTH domain family, member 1
• Masse moléculaire calculée: 559 aa, 61 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC050284
• Symbole du gène: YTHDF1
• Identification du gène (NCBI): 54915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.

Publications

Species	Application	Title
human	WB,RIP,IHC	Cancer Res; RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis; Authors - Danyu Chen
mouse	WB,IHC	Cell Mol Neurobiol; Wnt3a/YTHDF1 Regulated Oxaliplatin-Induced Neuropathic Pain Via TNF-α/IL-18 Expression in the Spinal Cord; Authors - Xiaohui Bai
human	WB	Cancer Med; BIRC5 facilitates cisplatin-chemoresistance in a m6 A-dependent manner in ovarian cancer; Authors - Yadan Fan
human	WB	Cancer Cell Int; DUSP5 regulated by YTHDF1-mediated m6A modification promotes epithelial-mesenchymal transition and EGFR-TKI resistance via the TGF-β/Smad signaling pathway in lung adenocarcinoma; Authors - Weina Fan ; KD Validated
mouse	WB,IHC	BMC Med; YTHDF1 in periaqueductal gray inhibitory neurons contributes to morphine withdrawal responses in mice; Authors - Chaopeng Ou

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