Anticorps Polyclonal de lapin anti-YTHDF2

YTHDF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 26771-1-AP

Synonymes

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2

---

Galerie de données de validation

• 

  WB analysis of Raji using 26771-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26771-1-AP (YTHDF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 26771-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26771-1-AP (YTHDF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26771-1-AP (YTHDF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 26771-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26771-1-AP (YTHDF2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 26771-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26771-1-AP (YTHDF2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HepG2 using 26771-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26771-1-AP (YTHDF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules Raji, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

26771-1-AP cible YTHDF2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YTHDF2 Protéine recombinante Ag25348
• Nom complet: YTH domain family, member 2
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC002559
• Symbole du gène: YTHDF2
• Identification du gène (NCBI): 51441
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.

Publications

Species	Application	Title
mouse	WB	J Neurochem; METTL14 is decreased and regulates m6 A modification of α-synuclein in Parkinson's disease; Authors - Honghu He
human	WB,IP,IF	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu ; KD Validated
		Clin Transl Med; N6-Methyladenosine modification mediated by METTL3 promotes DNA-PKcs expression to induce anlotinib resistance in osteosarcoma; Authors - Yining Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Yanhong (Verified Customer) (12-04-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HaCaT cells

YTHDF2 Antibody Western Blot, validation (1:1000 dilution) in HaCaT cells (Cat no:26771-1-AP)

{{ptg:RelatedPrimaryAntibodies}}