- Phare
- Validé par KD/KO
Anticorps Recombinant de lapin anti-YTHDF2
YTHDF2 Recombinant Antibody for WB, IF/ICC, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IF/ICC, IP, ELISA
Conjugaison
Non conjugué
CloneNo.
3L6
N° de cat : 81340-1-RR
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293, cellules C6, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Raji, cellules RAW 264.7 | 
| Résultats positifs en IP | cellules HeLa, | 
| Résultats positifs en IF/ICC | sodium arsenite treated HeLa cells, | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 | 
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
81340-1-RR cible YTHDF2 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris | 
| Hôte / Isotype | Lapin / IgG | 
| Clonalité | Recombinant | 
| Type | Anticorps | 
| Immunogène | YTHDF2 Protéine recombinante Ag5922 | 
| Nom complet | YTH domain family, member 2 | 
| Masse moléculaire calculée | 62 kDa | 
| Poids moléculaire observé | 65-70 kDa | 
| Numéro d’acquisition GenBank | BC002559 | 
| Symbole du gène | YTHDF2 | 
| Identification du gène (NCBI) | 51441 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par protéine A | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF2 antibody 81340-1-RR | Download protocol | 
| IF protocol for YTHDF2 antibody 81340-1-RR | Download protocol | 
| IP protocol for YTHDF2 antibody 81340-1-RR | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Nikolett (Verified Customer) (07-23-2025) | Antibody works well as 1:1000 for Western Blot, 1:300 for IHC and IF. For Western Blot I used various human lung cancer cell lines. For IHC and IF I used human NSCLC patient samples. 
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