Anticorps Polyclonal de lapin anti-YTHDF3-specific

• Phare
• Validé par KD/KO

YTHDF3-specific Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, RIP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

68

Avis (1)

N° de cat : 25537-1-AP

Synonymes

YTH domain family protein 3, YTH domain family, member 3, YTHDF3, YTHDF3-specific

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Galerie de données de validation

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  WB analysis of HeLa using 25537-1-AP

  WB result of YTHDF3-specific antibody (25537-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF3-specific transfected HeLa cells.

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  WB analysis using 25537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 25537-1-AP

  Immunofluorescent analysis of A549 cells using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HCT 116, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 18 publications below
• WB: See 59 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• CoIP: See 2 publications below
• RIP: See 10 publications below

Informations sur le produit

25537-1-AP cible YTHDF3-specific dans les applications de WB, RIP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YTHDF3-specific Protéine recombinante Ag22374
• Nom complet: YTH domain family, member 3
• Masse moléculaire calculée: 585 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC052970
• Symbole du gène: YTHDF3
• Identification du gène (NCBI): 253943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

Publications

Species	Application	Title
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human	WB,CoIP	Mol Cancer; A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis; Authors - Kaixuan Zeng ; KD Validated
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	WB	Nat Commun; Circular RNA encoded MET variant promotes glioblastoma tumorigenesis; Authors - Jian Zhong ; KD Validated
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio
human	IF	Nat Chem Biol; m6A-binding YTHDF proteins promote stress granule formation.; Authors - Ye Fu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Robert (Verified Customer) (01-17-2019)

1. scrambled siRNA2. YTHDF3 siRNA

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

YTHDF3-specific Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:25537-1-AP)