Anticorps Polyclonal de lapin anti-YTHDF3-specific

• Phare
• Validé par KD/KO

YTHDF3-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(119)

Avis (3)

N° de cat : 25537-1-AP

Synonymes

YTH domain family protein 3, YTH domain family, member 3, YTHDF3, YTHDF3-specific

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Galerie de données de validation

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  WB analysis of HeLa using 25537-1-AP

  WB result of YTHDF3-specific antibody (25537-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF3-specific transfected HeLa cells.

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  WB analysis using 25537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25537-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25537-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 25537-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using YTHDF3-specific antibody (25537-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HCT 116, cellules HeLa, cellules HepG2, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 25 publications below
• WB: See 98 publications below
• IHC: See 6 publications below
• IF: See 12 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• RIP: See 17 publications below

Informations sur le produit

25537-1-AP cible YTHDF3-specific dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: YTHDF3-specific Protéine recombinante Ag22374
• Nom complet: YTH domain family, member 3
• Masse moléculaire calculée: 585 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC052970
• Symbole du gène: YTHDF3
• Identification du gène (NCBI): 253943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

Publications

Species	Application	Title
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human	WB,CoIP	Mol Cancer; A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis; Authors - Kaixuan Zeng ; KD Validated
mouse	WB,RIP	Cell Mol Immunol; Inhibition of METTL3 in macrophages provides protection against intestinal inflammation; Authors - Huilong Yin
human	WB,IF	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	WB	Nat Commun; Circular RNA encoded MET variant promotes glioblastoma tumorigenesis; Authors - Jian Zhong ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-22-2024)

Antibody works in WB, ICC and IHC. ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Cytoplasmic staining and recognises stress granules. WB with antibody diluted in 4% milk in TBST, ON incubation. Also gave what looks like specific signal in mouse spinal cord (IHC, both DAB and fluorescence).

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa and SH-SY5Y cells/human; mouse tissue

YTHDF3-specific Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in HeLa and SH-SY5Y cells/human; mouse tissue (Cat no:25537-1-AP)

FH

Roy (Verified Customer) (06-15-2024)

Strong signal for YTHDF3 with this antibody from HeLa extracts (1h incubation at room temperature, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Robert (Verified Customer) (01-17-2019)

1. scrambled siRNA2. YTHDF3 siRNA

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

YTHDF3-specific Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:25537-1-AP)

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