Anticorps Polyclonal de lapin anti-ZBP1

• Phare
• Validé par KD/KO

ZBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(17)

Avis (1)

N° de cat : 13285-1-AP

Synonymes

C20orf183, DAI, DLM 1, DLM1, Tumor stroma and activated macrophage protein DLM-1

---

Galerie de données de validation

• 

  WB analysis using 13285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13285-1-AP (ZBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13285-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (13285-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

13285-1-AP cible ZBP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZBP1 Protéine recombinante Ag4119
• Nom complet: Z-DNA binding protein 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 42-68 kDa
• Numéro d’acquisition GenBank: BC028218
• Symbole du gène: ZBP1
• Identification du gène (NCBI): 81030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

Publications

Species	Application	Title
human	WB	J Med Virol; SARS-CoV-2 infection of polarized human airway epithelium induces necroptosis that causes airway epithelial barrier dysfunction; Authors - Siyuan Hao
human	WB	Cell Death Dis; Sensing of endogenous retroviruses-derived RNA by ZBP1 triggers PANoptosis in DNA damage and contributes to toxic side effects of chemotherapy; Authors - Fang Wang
human	IHC	Cell Death Dis; ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury; Authors - Xuechan Bi
rat,human	WB	Biochim Biophys Acta Mol Basis Dis; Ischemia-reperfusion injury induces ZBP1-dependent PANoptosis in endothelial cells; Authors - Yue Cui
human	WB	J Agric Food Chem; Integrative Gut Microbiota and Metabolomic Analyses Reveal the PANoptosis- and Ferroptosis-Related Mechanisms of Chrysoeriol in Inhibiting Melanoma; Authors - Yuxi Liu
human	WB	Sci Rep; Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells.; Authors - Bartosz Szczesny ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Charity (Verified Customer) (02-15-2024)

this will probably work at a lower concentration but I have not tried it yet.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse lung tissue sections

ZBP1 Antibody Immunofluorescence validation (1:100 dilution) in mouse lung tissue sections (Cat no:13285-1-AP)

{{ptg:RelatedPrimaryAntibodies}}