Anticorps Polyclonal de lapin anti-ZBP1

• Phare
• Validé par KD/KO

ZBP1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 13285-1-AP

Synonymes

C20orf183, DAI, DLM 1, DLM1, Z DNA binding protein 1, ZBP1

---

Galerie de données de validation

• 

  WB analysis using 13285-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13285-1-AP (ZBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13285-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

13285-1-AP cible ZBP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZBP1 Protéine recombinante Ag4119
• Nom complet: Z-DNA binding protein 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 42-68 kDa
• Numéro d’acquisition GenBank: BC028218
• Symbole du gène: ZBP1
• Identification du gène (NCBI): 81030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

Publications

Species	Application	Title
human	WB	Sci Rep; Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells.; Authors - Bartosz Szczesny ; KD Validated
human,mouse	WB	Physiol Genomics; PANoptosis in vascular smooth muscle cells regulated by TNF-α/IL-1β can be a new target for alleviating the progression of abdominal aortic aneurysm; Authors - Kun Li
human		J Biol Chem; Oxidative stress induces Z-DNA-binding protein 1-dependent activation of microglia via mtDNA released from retinal pigment epithelial cells.; Authors - Jamal Saada
human	WB	Toxicol Appl Pharmacol; The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells; Authors - Jun Li
human,mouse	WB	mBio; Sperm-Associated Antigen 9 Promotes Influenza A Virus-Induced Cell Death via the c-Jun N-Terminal Kinase Signaling Pathway.; Authors - Rui Gui
human	IHC	Am J Physiol Gastrointest Liver Physiol; DNA sensor associated type I Interferon signalling is increased in ulcerative colitis and induces JAK-dependent inflammatory cell death in colonic organoids; Authors - Peter Flood

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Charity (Verified Customer) (02-15-2024)

this will probably work at a lower concentration but I have not tried it yet.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse lung tissue sections

ZBP1 Antibody Immunofluorescence validation (1:100 dilution) in mouse lung tissue sections (Cat no:13285-1-AP)