Anticorps Recombinant de lapin anti-ZC3H12A

ZC3H12A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241876H5

N° de cat : 84521-5-RR

Synonymes

MCPIP, MCP-induced protein 1, MCP induced protein 1, Endoribonuclease ZC3H12A, EC:3.1.-.-

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Galerie de données de validation

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  WB analysis using 84521-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84521-5-RR (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 84521-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZC3H12A antibody (84521-5-RR, Clone: 241876H5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84521-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ZC3H12A Recombinant antibody (84521-5-RR, Clone:241876H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84521-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84521-5-RR against Human ZC3H12A were performed. The affinity constant is 6.27 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules MDA-MB-468, cellules Raji
• Résultats positifs en IF/ICC: cellules U-251,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84521-5-RR cible ZC3H12A dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZC3H12A Protéine recombinante Ag13877
• Nom complet: zinc finger CCCH-type containing 12A
• Masse moléculaire calculée: 599 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC005001
• Symbole du gène: ZC3H12A
• Identification du gène (NCBI): 80149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

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