Anticorps Polyclonal de lapin anti-ZCCHC6

• Phare
• Validé par KD/KO

ZCCHC6 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 25196-1-AP

Synonymes

HS2, KIAA1711, PAPD6, Terminal uridylyltransferase 7, TUT7, TUTase 7, ZCCHC6

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Galerie de données de validation

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  WB analysis of PC-3 using 25196-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25196-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25196-1-AP (ZCCHC6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25196-1-AP

  IP Result of anti-ZCCHC6 (IP:25196-1-AP, 4ug; Detection:25196-1-AP 1:1000) with HeLa cells lysate 1800ug.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293T, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

25196-1-AP cible ZCCHC6 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZCCHC6 Protéine recombinante Ag18240
• Nom complet: zinc finger, CCHC domain containing 6
• Masse moléculaire calculée: 1495 aa, 171 kDa
• Poids moléculaire observé: 171 kDa
• Numéro d’acquisition GenBank: BC032456
• Symbole du gène: ZCCHC6
• Identification du gène (NCBI): 79670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.

Publications

Species	Application	Title
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
human	WB	Mol Cell; 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.; Authors - Acong Yang ; KO Validated
human	WB	Mol Cell; A Mechanism for microRNA Arm Switching Regulated by Uridylation.; Authors - Haedong Kim ; KD Validated
human	WB,IP	Nat Commun; TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance; Authors - Acong Yang
human	WB,CoIP	Nat Commun; AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2.; Authors - Acong Yang
human	WB	Elife; The molecular basis of coupling between poly(A)-tail length and translational efficiency.; Authors - Kehui Xiang

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