Anticorps Polyclonal de lapin anti-ZDHHC5

• Phare
• Validé par KD/KO

ZDHHC5 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(21)

N° de cat : 21324-1-AP

Synonymes

DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5, Zinc finger DHHC domain-containing protein 5

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Galerie de données de validation

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  WB analysis of mouse kidney using 21324-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21324-1-AP

  WB result of ZDHHC5 antibody (21324-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZDHHC5 transfected HeLa cells.

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  WB analysis of HEK-293 using 21324-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21324-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21324-1-AP (ZDHHC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21324-1-AP

  IP result of anti-ZDHHC5 (IP:21324-1-AP, 4ug; Detection:21324-1-AP 1:500) with HEK-293 cells lysate 1680 ug.

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  IF Staining of HeLa using 21324-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21324-1-AP

  Immunofluorescent analysis of Hela cells, using ZDHHC5 antibody 21324-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HT-29 using 21324-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using ZDHHC5 antibody (21324-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 21324-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZDHHC5 (21324-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules HeLa, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IF/ICC: cellules HeLa, cellules HT-29
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

21324-1-AP cible ZDHHC5 dans les applications de WB, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZDHHC5 Protéine recombinante Ag14891
• Nom complet: zinc finger, DHHC-type containing 5
• Masse moléculaire calculée: 715 aa, 78 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC026967
• Symbole du gène: ZDHHC5
• Identification du gène (NCBI): 25921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.

Publications

Species	Application	Title
human	WB	Nature; ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D; Authors - Gang Du
human	WB,IP	Cell Death Differ; Palmitoylation restricts SQSTM1/p62-mediated autophagic degradation of NOD2 to modulate inflammation.; Authors - Lingli Zhou ; KD Validated
human	WB	Cell Death Dis; ZDHHC8 critically regulates seizure susceptibility in epilepsy.; Authors - Qin Yang
human	WB,IP,IF	Sci Rep; DHHC5-mediated palmitoylation of S1P receptor subtype 1 determines G-protein coupling.; Authors - Shaymaa Mohamed Mohamed Badawy ; KD Validated
mouse	WB,CoIP	Mol Neurobiol; AKAP150 and its Palmitoylation Contributed to Pain Hypersensitivity Via Facilitating Synaptic Incorporation of GluA1-Containing AMPA Receptor in Spinal Dorsal Horn.; Authors - Yinxia Li
	WB,IF	J Virol; Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation; Authors - Qisheng Qian

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