Anticorps Polyclonal de lapin anti-ZEB1

• Phare
• Validé par KD/KO

ZEB1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

199

Avis (2)

N° de cat : 21544-1-AP

Synonymes

AREB6, BZP, DELTA EF1, delta EF1,, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL2A, TCF 8, TCF8, Transcription factor 8, ZEB, ZEB1, ZFHEP, ZFHX1A

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Galerie de données de validation

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  WB analysis using 21544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21544-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21544-1-AP (ZEB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 21544-1-AP

  IP result of anti-ZEB1(IP:21544-1-AP, 4ug; Detection:21544-1-AP 1:8000) with COLO 320 cells lysate 1320 ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules COLO 320, cellules MCF-7, cellules MDA-MB-453s, cellules PC-3, tissu de côlon de souris
• Résultats positifs en IP: cellules COLO 320,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 21 publications below
• WB: See 182 publications below
• IHC: See 24 publications below
• IF: See 16 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 10 publications below

Informations sur le produit

21544-1-AP cible ZEB1 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZEB1 Protéine recombinante Ag16204
• Nom complet: zinc finger E-box binding homeobox 1
• Masse moléculaire calculée: 1125 aa, 124 kDa
• Poids moléculaire observé: 190-210 kDa
• Numéro d’acquisition GenBank: BC112392
• Symbole du gène: ZEB1
• Identification du gène (NCBI): 6935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZEB1(zinc-finger-enhancer binding protein 1), which is a members of the Zincfinger-Homeobox (ZFH)-family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene . Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.

Publications

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA; Authors - Ya-Rui Hou
human	chIP	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
human	WB	Nat Commun; Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63.; Authors - Yingjie Liu
human	WB,IP,ChIP	Nat Commun; Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasis.; Authors - Nastaran Mohammadi Ghahhari
human	WB, ChIP	J Exp Med; Squamous cell carcinoma subverts adjacent histologically normal epithelium to promote lateral invasion.; Authors - Priyanka Singh
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kathryn (Verified Customer) (10-14-2021)

Antibody worked really well first time and produced clear Zeb1 bands at the MW expected, with barely any non specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVECs

FH

K (Verified Customer) (12-20-2020)

I used tis Ab for western blotting at 1:2000 dilution and got good results.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: human liver cells

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