Anticorps Polyclonal de lapin anti-SARA

• Phare
• Validé par KD/KO

SARA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 22033-1-AP

Synonymes

hSARA, Madh interacting protein, MADHIP, Novel serine protease, NSP, Receptor activation anchor, SARA, SMADIP, ZFYVE9

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Galerie de données de validation

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  WB analysis using 22033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22033-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22033-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22033-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22033-1-AP

  IP Result of anti-SARA (IP:22033-1-AP, 5ug; Detection:22033-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of human kidney using 22033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22033-1-AP (SARA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 22033-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22033-1-AP (SARA antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 22033-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SARA antibody (22033-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 22033-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SARA antibody (22033-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

22033-1-AP cible SARA dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARA Protéine recombinante Ag16929
• Nom complet: zinc finger, FYVE domain containing 9
• Masse moléculaire calculée: 1425 aa, 156 kDa
• Poids moléculaire observé: 156 kDa
• Numéro d’acquisition GenBank: BC032680
• Symbole du gène: ZFYVE9
• Identification du gène (NCBI): 9372
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZFYVE9, also known as SARA (Smad anchor for receptor activation), is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SARA functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization.

Publications

Species	Application	Title
human	WB	Structure; Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.; Authors - Deepankar Gahloth ; KD Validated
mouse	WB,IF	J Bone Miner Res; Dysfunction of Caveolae-mediated Endocytic TβRI Degradation Results in Hypersensitivity of TGF-β/Smad Signaling in Osteogenesis Imperfecta; Authors - Changgui Shi
human	WB,IF	Sci Adv; Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis; Authors - Zheng-Zhou Lu ; KO Validated