Anticorps Polyclonal de lapin anti-ZNF410

• Phare
• Validé par KD/KO

ZNF410 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ChIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 14529-1-AP

Synonymes

Zinc finger protein APA 1, Zinc finger protein 410, APA1, APA 1, Another partner for ARF 1

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Galerie de données de validation

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  WB analysis using 14529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14529-1-AP (ZNF410 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14529-1-AP (ZNF410 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, cellules U2OS, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• ChIP: See 1 publications below

Informations sur le produit

14529-1-AP cible ZNF410 dans les applications de WB, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF410 Protéine recombinante Ag6028
• Nom complet: zinc finger protein 410
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52-67 kDa
• Numéro d’acquisition GenBank: BC050683
• Symbole du gène: ZNF410
• Identification du gène (NCBI): 57862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNF410 (Zinc Finger Protein 410) is a transcription factor involved in the regulation of gene expression, particularly in the context of fetal hemoglobin (HbF) repression and chromatin remodeling. This protein is highly conserved among mammals and plays a crucial role in erythroid cells. The study found that ZNF410 uniquely silences HbF by activating its sole target gene, CHD4, a known HbF repressor in erythroid cells. (PMID: 40158221)

Publications

Species	Application	Title
human	WB	Nat Genet; ZNF410 represses fetal globin by singular control of CHD4.; Authors - Divya S Vinjamur ; KO Validated
human	WB	Mol Cell; ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression.; Authors - Xianjiang Lan
mouse	WB	EMBO J; PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.; Authors - Xixi Tao
human	WB	Mol Ther; Development of a double shmiR lentivirus effectively targeting both BCL11A and ZNF410 for enhanced induction of fetal hemoglobin to treat β-hemoglobinopathies.; Authors - Boya Liu
human	WB	Biomedicines; Heteronemin Suppresses Lymphangiogenesis through ARF-1 and MMP-9/VE-Cadherin/Vimentin.; Authors - Hsien-Lin Chen
mouse	WB	Free Radic Biol Med; Critical role of caveolin-1 in intestinal ischemia reperfusion by inhibiting protein kinase C βII; Authors - Zhao Chen

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