Anticorps Recombinant de lapin anti-ZO-2

ZO-2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230474H8

N° de cat : 83111-4-RR

Synonymes

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

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Galerie de données de validation

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  WB analysis using 83111-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 83111-4-RR

  C6 cells were subjected to SDS PAGE followed by western blot with 83111-4-RR (ZO-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 83111-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-2 antibody (83111-4-RR, Clone: 230474H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A431 using 83111-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ZO-2 Recombinant antibody (83111-4-RR, Clone:230474H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U2OS using 83111-4-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug ZO-2 Recombinant antibody (83111-4-RR, Clone:230474H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83111-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83111-4-RR against Human XRN2 were performed. The affinity constant is 80.7 pM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules HepG2, cellules MCF-7
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules A431, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83111-4-RR cible ZO-2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ZO-2 Protéine recombinante Ag13534
• Nom complet: tight junction protein 2 (zona occludens 2)
• Masse moléculaire calculée: 134 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC027592
• Symbole du gène: TJP2
• Identification du gène (NCBI): 9414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

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