Anticorps Monoclonal anti-ZO-1

• Phare
• Validé par KD/KO

ZO-1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

51

CloneNo.

1G4A1

N° de cat : 66452-1-Ig

Synonymes

Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1

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Galerie de données de validation

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  WB analysis using 66452-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66452-1-Ig (ZO-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66452-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66452-1-Ig (ZO-1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

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  IF Staining of human breast cancer using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ZO-1 antibody (66452-1-Ig, Clone: 1G4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MDCK using 66452-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 66452-1-Ig (ZO-1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules A431, cellules COLO 320, cellules HEK-293, cellules HeLa, cellules HepG2, cellules LNCaP
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules MDCK, tissu de cancer du sein humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 9 publications below
• IF: See 25 publications below

Informations sur le produit

66452-1-Ig cible ZO-1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZO-1 Protéine recombinante Ag16454
• Nom complet: tight junction protein 1 (zona occludens 1)
• Masse moléculaire calculée: 1748 aa, 195 kDa
• Poids moléculaire observé: 230 kDa
• Numéro d’acquisition GenBank: BC111712
• Symbole du gène: TJP1
• Identification du gène (NCBI): 7082
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).

Publications

Species	Application	Title
	WB	Sci Adv; Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage; Authors - Dayun Feng
human	WB,IF	Nat Commun; An electrochemical biosensor for the detection of epithelial-mesenchymal transition.; Authors - Xin Du
human	IF	Cell Death Differ; SARS-CoV-2 spike protein dictates syncytium-mediated lymphocyte elimination.; Authors - Zhengrong Zhang
human	IF	ACS Appl Mater Interfaces; Betaine-Based and Polyguanidine-Inserted Zwitterionic Micelle as a Promising Platform to Conquer the Intestinal Mucosal Barrier; Authors - Kedong Liu
human	IF	Int J Biol Macromol; zwitterionic Pluronic analog-coated PLGA nanoparticles for oral insulin delivery; Authors - Kedong Liu
	IF	Cell Mol Life Sci; Silencing circSERPINE2 restrains mesenchymal stem cell senescence via the YBX3/PCNA/p21 axis; Authors - Fenglei Chen